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DISCUSSION
The natural corneal stromas have already been used as the carrier for corneal cells in previous research, but these corneal stroma scaffolds contained cells [6,7,9]. The cell components are the source of the antigens of the major histocompatibility complex [10,11]. The natural corneal stroma containing cells is not fit for the standard of scaffold of tissue engineering cornea because of its antigens. One way to reduce the antigen and pathogenicity of the natural cornea stroma may be to remove the cell compositions in it.
However, decellularization protocols applied on the natural corneal stroma may affect the composition and morphology of the surface of the natural materials. This may affect the cell compatibility of the material.
Figure 1A:Phase contrast photograph shows the growth of the rabbits corneal epithelium cells on APCS at 3 days. (magnification ×200); B: The section of H&E shows the growth of the rabbits corneal epithelium cells on APCS in 23 layers at 10 days (magnification ×400); C: Phase contrast photograph shows the growth of the rabbits corneal stroma cells on APCS at 4 days (magnification ×200); D: The section of H&E shows the stroma cells invade into the interlaminar of scaffold at 5 days (magnification ×200)（略）
In this study, to observe the effect of the rabbits corneal cells growing on APCS, we performed the experiment in vitro. The results showed cornea epithelium cells could grow and stratify on the surface of APCS without any coating. The study also suggested that the corneal stromal cells could grow on APCS and invade the interlaminar of the scaffold. This indicates that APCS can support the growth of the rabbit corneal cells in vitro. We suppose that APCS prepared by the methods in accordance with previous report [8], like other acellular biologic materials, comprise the natural corneal ECM retaining many types of cytokines and chemical signals [12]. APCS can provide the surface such as the basement membrane for the growth of corneal epithelium and the porous microenvironment for the stromal cells to invade and proliferate [8].
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